最高学历：博士

职称：教授、硕士研究生导师

所属科室：口腔修复科

科研工作：

论文：

1. Lei KE, Wang L, Ma B, Shi P, Li L, Che T, He X. Effect of an EDA-A1 gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells. Exp Ther Med. 2016 Feb;11(2):535-539. Epub 2015 Dec 18. PubMed PMID: 26893642; PubMed Central PMCID: PMC4734069.

2. Jiang K, Che C, Ding Z, Zeng S, Wang W, He X. Precision diagnosis and antidiastole on supernumerary cusp of tooth by CBCT. Surg Radiol Anat. 2016 Feb [Epub ahead of print] PubMed PMID: 26861010.

3. Zhou J, Jiang N, Wang S, Hu X, Jiao K, He X, Li Z, Wang J. Exploration of Human Salivary Microbiomes-Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects. PLoS One. 2016 Jan 19;11(1):e0147039. doi: 10.1371/journal.pone.0147039. eCollection 2016. PubMed PMID: 26784334; PubMed Central PMCID: PMC4718657.

4. Chen B, Che T, Bai D, He X. [Effects of non-saccharomyces albicans metabolic products on the proliferation of human umbilical vein endothelial cell ECV304]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2013 Apr;31(2):186-90. Chinese. PubMed PMID:23662564.

5. Shi X, Chen J, Che T, Bai D, He X. [A study on human tongue cancer cells' proliferation affected by Lactobacillus acidophilus]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2012 Feb;30(1):87-92. Chinese. PubMed PMID: 22389975.

6. Chen J, He X, Wu L, Che T. [The correlation between oral colonization of Helicobacter pylori and gastrointestinal disease]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Aug;29(4):351-4. Chinese. PubMed PMID: 21932650.

7. Zhang L, Che TJ, Shi XY, He XY. [A study on human umbilical vein endothelial cell ECV304 proliferation induced by Saccharomyces albicans]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Jun;29(3):289-93. Chinese. PubMed PMID: 21776858.

8. Lei K, Che TJ, Wang JM, Deng N, Zhang L, He XY. [Mutation analysis of the eda-A1 gene for hypohidrotic ectodermal dysplasia and construction of recombined eukaryotic expression vector]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Dec;27(6):610-3. Chinese. PubMed PMID: 20077893. Lei K, He XY. [Hypohidrotic ectodermal dysplasia: a case report]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Jun;27(3):350-2. Chinese. PubMed PMID: 19637495.

9. He X, Shu W, Kang Y, Li Z, Zhang J, Kari K, Meurman JH. Esthetic and functional rehabilitation of a patient with nonsyndromic oligodontia: a case report from China. J Esthet Restor Dent. 2007;19(3):137-42; discussion 143. PubMed PMID: 17518902.

1. DENG Ni, ZHANG Jinting, ZHANG Lin, CHE Tuanjie, KANG Yanli, FAN Zhen, HE Xiangyi

Epidemiologic study on Candida species transmission from mother to their neonate in Lanzhou J Pract Stomatol 2009Vol.25(6)Nov,25

[Abstract] Objective: To investigate the candidal infection status in pregnant women in Lanzhou, and candidal transmission from mothers to their newborn infants. Methods: Vaginal fluid and saliva samples from 104 puerperas, as well as 104 saliva samples from their newborn infants, were collected. The candida species were cultured, isolated and identified using CHROMagar media. Further identification was taken using molecular biological technology. Result: In 81 of 312 specimens (104×2 from mothers and 104 from infants), candida species were found positively. 39.42%(41 cases)and 33.65%(35 cases) were observed in the vaginal and oral of puerperas respectively, and 21.15%(22 cases) in both vagina and oral cavity. The rate 4.81%(5 cases) was found in oral cavities of newborn infants. The distribution of candida species in positive specimens were 53 Candida albicans, 33 Candida glabrata, 2 Candida krusei and 1 Candida tropical. In 2 pairs of mother-infant, the same genotype of Candida ablicans was identified using PCR method. Conclusion: The Candida isolation rate of newborn infants and transmission rate from mother to their neonate in Lanzhou are higher than that in reported other areas. The colonization of candida in newborn infants is relevant to both horizontal and vertical transmission. It can decrease the likelihood of Candidal infection in newborn infants by controlling the Candidal transmission in hospital and preventing the infection in pregnant women.

[Key Word] Candida, pregnant women, newborn infant, oral, epidemiology

2. LEI Ke, CHE Tuan-jie, WANG Jing-ming, DENG Ni, ZHAGN Lin, HE Xiang-yi.

Mutation analysis of the eda-A1 gene for hypohidrotic ectodermal dysplasia and construction of recombined eukaryotic expression vector West China Journal of Stomatology Vol.27 No.6 Dec.2009

[Abstract] Objective The purpose of this study was to clone and analyze mutation in the eda-A1 gene for hypohidrotic ectodermal dysplasia (HED), and to construct a new recombined eukaryotic expression vector (mutant M, wild W) as a basis for further study on the genetic function. Methods After total mRNA was extracted from peripheral blood lymphocytes from the HED affect patient and control, eda-A1 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) with a pair of specific primers containing the constriction enzyme sites of BamHⅠ and HindⅢ. When vector pcDNA3.1(-) and eda-A1 (M/W) were digested by BamHⅠ and HindⅢ respectively, eda-A1(M/W) fragment was then ligated to vector pcDNA3.1(-) and the new vector was named as pcDNA3.1(-)-eda-A1-M/W. Results eda-A1 gene was successfully cloned and a novel missence mutation (1091T→C) was identified, which changes the codon 306 from glutamine to proline; PCR, restrictive endonuclease analysis and  DNA sequencing were then performed to identify the recombinant eukaryotic expression vector pcDNA3.1(-)-EDA-A1-M/W, and the results were surely confirmed. Conclusion Our result indicates that the novel missense mutation in EDA is associated with the isolated tooth agenesis and provide preliminary explanation for the abnormal clinical phenotype at a molecular structural level. And also, the recombinant eukaryotic expression vector pcDNA3.1(-)-eda-A1-M/W was successfully constructed, which will be thereafter taken use of in further study on eda gene in odontogenesis.

3. Jinting Zhang, Tuanjie Che, Xiangyi He. In Vitro Antibiotic Effect of the Glycyrrhizae and Astragalus Leaching Solution on Streptococcus mutant. Shanxi Journal of Traditional Chinese Medicine, 2009,30;3:341-344

Abstract

[Abstract] Objective: To study the antibiotic effect of the leaching solution of glycy- rrhizae and astragalus on one of the cariogenic bacteria -- Mutans streptococci and to compare to that of the oral bacteriostatic product from foreign country (MI). Methods: Glycyrrhizae and astragalus from Gansu was used as the raw material. Mutans streptococci were chosen as the experimental isolates that were cultured in the medium for 24 hours. The PH and OD600 values were then measured. Statis tical analysis was conducted using SPSS13.0. Result: The leaching solutions of glycyrrhizae and astragalus have inhibitory effects respectively on the growth and acid production of mutans streptococci, and have no significant differences com- paring that of foreign bacteriostatic product. Conclusion: The leaching solution of glycyrrhizae and astragalus respectively has antibacterial effect on Mutans strep- tococci.

[Key words] Glycyrrhizae; Astragalus; Streptococcus mutan; Acid production

4. Yan Zheng, Zhenya Li, Xiangyi He, Baocheng Cao. The effects of fixed orthodontic appliances on oral pathogenic yeasts of adolescence. Journal of practical stomatology, 2008,3:401-405

Abstract

Objective: To compare the oral Candidas’presence and variance before and during treatment of fixed orthodontic appliances ( FOA) on teenagers.

Methods: The microorganism samples were taken before and after orthodontic treatment by culture of identification and molecular biology techniques through self2controlled method. Thus, the effects of orthodontic appliance on oral pathogenic yeasts were longitudinally studied.

Results: The percentage of people who carried some kinds of Candidas and the sum of colony forming unit was increased obviously after orthodontic treatment for 2 months. The sort of oral Candidas before and after treatment was also changed obviously.

Conclusion: Fixed orthodontic appliances can influence oral pathogenic yeasts in adolescence.

5. Jinting Zhang, Ni Deng, Tuanjie Che, Yanli Kang, Xiangyi He. In vitro antibiotic effect of the leaching solution of astragalus on cariogenic bacteria. Journal of practical stomatology 2009,4:588-590

[Abstract]　Objective: To study the antibiotic effect of the leaching solution of astragalus on the cariogenic bacteria and to compare to that of the oral bacteriostatic product from foreign country (MI). Methods: Astragalus from Gansu was used as the raw material. Mutans streptococci and Lactobacilli were chosen as the experimental isolates that were cultured in the medium for 24 hours. The PH and OD600 values were then measured. Statistical analysis was conducted using SPSS13.0. Result: The leaching solution of astragalus has inhibitory effects on the growth and acid production of mutans streptococci and lactobacilli, and have no significant difference comparing that of foreign bacteriostatic product. Conclusion: The leaching solution of astragalus has antibacterial effect on cariogenic bacteria, which is equal to that of MI.

[Key words] Leaching solution of astragalus (lSA); Streptococcus mutan; Lactobacillus; Acid production

6. Lin Zhang, Xiangyi He. Research progress and prospects in oral health impact profile. Chinese Journal of Conservative Dentistry. 2008, 18 (8) : 476-480

Abstract

With the chang of medicinal pattern, research on psychological factors impacting diseases has become an important content in modern medicine. Oral health impact profile (OHIP) is subjected to the comprehensive assessment of oral diseases in the areas of physical, psychological and social function. It is well complementary for modern dentistry researches, and also leads to the much concerns on oral health related quality in the world. To explore the possibility of OHIP application in China, this paper aimed to describe the formation, development, features of OHIP, and to express the prospects in the near future of OHIP in Chinese.

7. Jianye Zhou, Jinting Zhang, Yun Xiao, Tuanjie Che, Xiangyi He. The Cariogenicity by MS and LB Isolated from the elderly’s Decayed Tooth. Chinese Journal of Gerontology. 2009,5,29:1258-1259

Abstract

Objective: To study the cariogenic characteristics of Mutans Streptococci (MS) and Lactobacilli (LB) during the cariogenic development of the elderly. Methods: 136 dental carious samples were collected for isolation, cultivation, identification. CFU count was then conducted in the sorts by age, carious degree and carious activity. The mean count of MS and LB’s CFU, and the samples’ amount resulted from the questioned bacterium (bacteria) were statistically analyzed by SPSS 13.0 after logarithmic conversion to data. Results: 1, There was a significant difference in mean counts of SM and LB in the group of age, carious degree and carious activity, respectively (P＜0.05). 2, There was a significant difference in the amount of commonly cariogenic samples by SM and LB in the elderly group (P＜0.05), which was closely correlated with the carious degree (P＜0.05). Conclusion: MS and LB are mutualism in the cariogenic development of the elderly, the median caries, and the serious caries respectively.

[Key words] Mutans Streptococci; Lactobacilli; the elderly; dental caries

8. Ke Lei, Xiangyi He. Research progress in the genes and proteins of hypohidrosis ectodermal dysplasia. International Journal of Stomatology. 2009,1,Vol.36:120-122

Abstract

Hypohidrosis ectodermal dysplasia (HED) is a rare congenital disorder that results in abnormalities in the structures of ectodermal origin tissues, such as teeth, eccrine sweat glands, hair and nails. However, most of studys in HED is presented in the form of clinic case reports. This article reviews the research progress of HED in types, genetic locations, proteins, and signaling pathways in odontogenesis.

9. Ke Lei, Xiangyi He.  Hypohidrotic ectodermal dysplasia: A case report. West China Journal of Stomatology. 2009,3,Vol.27:350-352

Abstract

Hypohidrotic ectodermal dysplasia（HED） is a hereditary disorder characterized by abnormal development of tissues derived from ectoderm. A case of hypohidrotic ectodermal dysplasia was reported, and the molecular biological progresses in this area were reviewed.